Determination of pathogens and contamination indicatorsin fresh pork sausages in the state of São Paulo / Determinação de patógenos e indicadores de contaminação em linguiças frescais de origem suína no estado de São Paulo

The highest cost of beef and chicken meat justifies higher consumption of fresh sausage products, especially linguiça, due to their easy preparation and affordability for consumers, making necessary an evaluation of sanitary hygienic conditions of these products. The objective was to investigate the presence of pathogens such as Salmonella spp., coagulase-positive Staphylococcus, thermotolerant coliforms, Escherichia coli and Campylobacter spp. in artisanal and inspected fresh pork sausages. It was found that 12% (6/50) of the artisanal sausage samples were contaminated with Salmonellaspp., 58% (29/50) presented coagulase-positive Staphylococcus levels above the acceptable limits for consumption and 76% (38/50) presented thermotolerant coliform levels above the acceptable limits. In sausage samples produced under inspected conditions, 6% (3/50) were contaminated with Salmonella spp., 24% (12/50) presented thermotolerant coliform levels above the acceptable limits, 2% (1/50) presented enteropathogenic E. coli. None samples showed coagulase-positive Staphylococcus counts above the limits, or presence of Campylobacter spp. Sensitizing traders and consumers about the importance of inspection service in food of animal origin is urgent for a sanitary acceptable production, since foodborne diseases continue to be a public health problem.

O alto custo das carnes de frango e bovina justifica maior consumo de produtos embutidos frescais, especialmente a linguiça, devido ao preço acessível ao consumidor e fácil preparo, tornando necessário estudos para avaliar as condições higiênico-sanitárias. Os objetivos foram verificar a presença de Salmonella spp., Staphylococcus coagulase positiva, coliformes termotolerantes, Escherichia colipatogênicas e Campylobacter spp. em linguiças suínas tipo frescal artesanais e fiscalizadas. Os resultados obtidos em linguiças artesanais foram de 12% (6/50) contaminadas com Salmonella spp., 58% (29/50) de Staphylococcus coagulase positiva e 76% (38/50) nas quantificações de coliformes termotolerantes, dados que apresentam níveis de contaminação superiores aos da legislação vigente. Nas linguiças produzidas sob fiscalização detectou-se 6% (3/50) de contaminação por Salmonella spp.; 24% (12/50) de quantificação de coliformes termotolerantes acima dos limites aceitáveis, e 2% (1/50) de E. coli enteropatogênica. Nenhuma amostra apresentou contagens de Staphylococcus coagulase positiva fora dos padrões, ou contaminação por Campylobacter spp. Sensibilizar comerciantes e consumidores sobre a importância do serviço de inspeção em alimentos de origem animal é premente para que haja uma produção sanitária aceitável, pois doenças transmitidas por alimentos continuam sendo um problema à saúde pública.

Cross-cultural invariance of the Spanish version of the COVID-19 Assessment Scorecard to measure the perception of government actions against COVID-19 in Latin America

Abstract Objectives The present study aimed to evaluate the measurement invariance of a general measure of the perception of governmental responses to COVID--19 (COVID-SCORE-10) in the general population of 13 Latin American countries. Methods A total of 5780 individuals from 13 Latin American and Caribbean countries selected by non-probabilistic snowball sampling participated. A confirmatory factor analysis was performed and the alignment method was used to evaluate invariance. Additionally, a graded response model was used for the assessment of item characteristics. Results The results indicate that there is approximate measurement invariance of the COVID-SCORE-10 among the participating countries. Furthermore, IRT results suggest that the COVID-SCORE-10 measures with good psychometric ability a broad spectrum of the construct assessed, especially around average levels. Comparison of COVID-SCORE-10 scores indicated that participants from Cuba, Uruguay and El Salvador had the most positive perceptions of government actions to address the pandemic. Thus, the underlying construct of perception of government actions was equivalent in all countries. Conclusion The results show the importance of initially establishing the fundamental measurement properties and MI before inferring the cross-cultural universality of the construct to be measured.

Recursos Públicos na Esfera Privada: Política de Educação Especial em um Município Paulista de Grande Porte / Public Resources in the Private Sphere: Special Education Policy in a Large Municipality of São Paulo

RESUMO: A Educação Especial brasileira tem marcas históricas da privatização, uma delas é focalizada neste artigo, que tem por objetivo analisar o repasse de recursos financeiros públicos da educação para a esfera privada em um município paulista de grande porte, entre 2012 e 2017. Adotando abordagem qualitativa, são analisados os valores liquidados em favor da Educação Especial direcionados à esfera privada a partir de fontes documentais, abrangendo contratos com empresas terceirizadas, termos de colaboração e aditamento, relatórios de remuneração de profissionais da Educação Especial e documentos de repasse de recursos à esfera privada. Os resultados indicam aumento dos recursos destinados a essa modalidade na esfera privada em decorrência, principalmente, da necessidade de contratação de cuidadoras(es), até aquele momento sem provimento de cargo nessa rede de ensino. Também expressam que a inclusão escolar reproduz modos de financiamento que marcam a história da Educação Especial no Brasil. A parceria com instituições privadas sem fins lucrativos e a contratação de empresas terceirizadas vão ao encontro da perspectiva gerencial da administração pública. No caso analisado, por um lado, a política de inclusão escolar terceirizou a manutenção dos serviços a partir da concessão para a esfera privada; por outro lado, houve crescente aumento do financiamento do setor público municipal.

ABSTRACT: Brazilian Special Education has historical marks of privatization, one of which is focused on this article, which aims to analyze the transfer of public financial resources from education to the private sphere in a large municipality in São Paulo, between 2012 and 2017. Adopting a qualitative approach, the amounts settled in favor of Special Education directed to the private sphere are analyzed based on documentary sources, covering contracts with outsourced companies, terms of collaboration and additions, reports on remuneration of Special Education professionals and documents for the transfer of funds to the private sphere. The results indicate an increase in resources allocated to this modality in the private sphere, mainly due to the need to hire caregivers, until that moment without filling a position in this teaching network. They also express that school inclusion reproduces funding modes that mark the history of Special Education in Brazil. The partnership with non-profit private institutions and the contracting of outsourced companies are in line with the managerial perspective of public administration. On the one hand, in the analyzed case, the school inclusion policy outsourced the maintenance of services from the concession to the private sphere, on the other hand, there was a growing increase in funding from the municipal public sector.

Characterization of primary direct-acting antiviral (DAA) drugs resistance mutations in NS5A/NS5B regions of hepatitis C virus with genotype 1a and 1b from patients with chronic hepatitis

ABSTRACT The Hepatitis C virus (HCV) infection is a public health problem. The high level of HCV replication and its lack of post-transcriptional correction mechanisms results in the emergence of viral variants and the difficulty in determining polymorphisms and variants that contain the substitutions associated with resistance towards new antivirals. The main focus of this study was to map the NS5A and NS5B polymorphisms and resistance mutations to new antiviral drugs in HCV strains genotype 1 from patients with chronic hepatitis C infection. Serum samples were collected from patients who underwent routine viral load tests at the Instituto Adolfo Lutz, Sao Paulo city, Brazil. A total of 698 and 853 samples were used for the characterization of NS5A and NS5B regions, respectively, which comprise the HCV genotypes 1a and 1b. The prevalence of resistance mutations found in the NS5A region was 6.4%, with Y93H, L31M, Q30R, and Y93N as the main resistance-associated substitutions (RAS). No NS5B-associated RAS was observed for any of the analyzed drugs. These findings support that the RAS test should be offered to individuals with poor response to double combination regimens prior to treatment initiation, thereby assisting strain vigilance and selection of effective treatment or retreatment options using DAA regimens.

Validation of the brief scale for the evaluation of acculturation stress in migrant population (EBEA)

Abstract Background: Acculturation stress is associated with poorer physical and mental health and a lower level of psychological well-being. The causes of acculturation stress are diverse, but most are similar in the migrant population. Despite the importance of evaluating this variable, few studies have reported culturally adapted and validated instruments for specific populations. Based on this, the aim of this study was to evaluate the psychometric properties of a short scale for the evaluation of acculturation stress (EBEA). Methods: Two studies were conducted, involving 1725 first-generation Colombian and Peruvian migrants living in Chile, between the ages of 18 and 60 years. In addition to the EBEA and as evidence of validity, the Beck Anxiety Scale and the WHOQOL-BREF psychological health domain were applied. A confirmatory factor analysis was carried out, and the reliability and nomological validity were evaluated. Results: The results in both studies indicated that the scale presents a factorial structure of three dimensions: (a) the stress derived from the preparation and departure from the country of origin, (b) the stress produced by socioeconomic concerns in the host country, and (c) the tensions typical of adaptation to sociocultural changes or Chilean society. The reliability coefficients and the analysis of their nomological validity were very good. Conclusions: The EBEA is a measure that offers quick, useful screening for researchers who need a short measure for research among migrants. This tool contributes to the work of education, prevention, and intervention in the field of general health and migrants' mental health.

Funciones ejecutivas entre músicos y no músicos: un metaanálisis / Executive functions between musicians and non-musicians: a metanalysis

Resumen Se ha sugerido que la práctica profesional de un instrumento musical esta relacionada con mejores puntajes en tareas de funciones ejecutivas. Con el fin de examinar sistemáticamente esta hipótesis se realizó un metaanálisis para identificar el efecto de la interpretación de un instrumento en las funciones ejecutivas, así como las variables que podrían moderar dicho efecto. El metaanálisis incluyó estudios empíricos que: (1) compararon los puntajes en medidas de funciones ejecutivas entre músicos y no músicos; (2) reportaron las medias y desviaciones estándar; (3) el grupo de comparación no recibió entrenamiento musical; y (4) los participantes no presentaron discapacidad motora o cognitiva. Se identificó que los músicos, en comparación con los no músicos, muestran mejores puntajes en tareas de funciones ejecutivas (d = .712). Los resultados apoyan la hipótesis de que la práctica de un instrumento musical está relacionada con mejores puntuaciones en tareas de funciones ejecutivas.

Abstract Nowadays, it is widely accepted that experience can modify multiple aspects of the functioning and structure of the brain; however, there is still a long way to go to understand the mechanisms behind these modifications. In order to understand the relationship between experience and brain plasticity, models and paradigms have been adapted from cognitive neurosciences; one of them has been music training. In the last decades, this paradigm has strengthened because it allows us to understand the structural and functional modifications produced by training. It has been demonstrated that musicians compared to non-musicians present larger sizes of specific brain regions, in particular, the prefrontal cortex, which is associated with a better performance in executive functions tasks. In addition, long-term neurophysiological changes have been identified in people who play an instrument. A relationship between playing an instrument and better performance in executive functions has been identified. However, the evidence of this effect is contradictory. Executive functions are a series of general control processes that help regulate thinking and behavior. Some research indicates that learning an instrument is associated with better performance in working memory, but not regarding tasks related to inhibitory control and cognitive flexibility. However, other studies have revealed significant differences between musicians and non-musicians, both in working memory and tasks of cognitive flexibility as well as in inhibitory control. It is possible that the contradictory results are due to the methodological diversity through which this topic has been addressed, since some studies have carried out experimental and quasi-experimental designs, and others have developed correlational approaches. In addition, different theoretical models have been used to evaluate executive functions. For this study, we work with the theoretical model of Miyake et al. (2000), which is a solid theoretical-empirical proposal about three clearly differentiable executive functions: inhibitory control, cognitive flexibility, and working memory. A meta-analysis was performed with the purpose of identifying the effect of playing an instrument on executive functions as well as identifying the moderator variables. By doing this, we wanted to clarify the problem in question and the controversial results; therefore, empirical studies comparing musicians against non-musicians were included. The comparison group should not have been exposed to musical training, the participants should not present motor or cognitive disabilities, and finally, studies should report means and standard deviations for the scores on executive functions tasks. We included papers published between January 2000 and July 2017. The moderator variables studied were: age in months at the time of evaluation, age at which training began, and total years of training. The coding strategy allowed us to identify a total of 42 294 articles. The titles and abstracts were analyzed and those that did not meet the inclusion criteria were eliminated. This procedure allowed the detection of 24 articles for future analysis, and 12 out of them were finally selected. These 12 articles contributed to 60 effect sizes and 513 participants. The results showed that musicians have a better performance in executive functions tasks when they are compared to non-musicians, d = .712, n = 60, IC95% = (.57; .85), p < .0001. Additionally, the analysis of moderating variables revealed that the practice of an instrument equally benefits all executive functions, and the most important benefit occurs in older adults and children. Results support the hypothesis that the high level of difficulty involved with learning to play an instrument can influence a better performance in executive functions tasks. Our study points out the need of designing research that allows the scientific community to investigate the effect of prolonged musical training over time.

Assessment of pre- and post-operative nutritional status in patients undergoing orthognathic surgery / Avaliação do estado nutricional pré e pós-operatório em pacientes submetidos à cirurgia ortognática

ABSTRACT: Introduction: The maxillofacial procedures for skeletal deformities are characterized by structural-morphological changes derived from unfavorable genetics with diagnosis performed during craniofacial growth. Orthognathic surgery requires a total restriction on chewing for 60 days, leading to loss of body weight in overweight and obese individuals, as well as in well-nourished patients. Objective: Evaluating the pre- and post-operative nutritional status of patients undergoing orthognathic surgery. Material and methods: This is an interventional study. The study group received supplementation with whey protein, L-arginine, L-glutamine, and fatty acid Omega-3, and both groups were assessed in terms of anthropometric and biochemical measurements. The evaluation of soft tissue healing was conducted after surgery. Results: It is possible to infer that the percentage of skeletal muscle mass tends to increase in the intervention group, however, the weight loss was greater (-4.88%). Uric acid increased post-operative in the control group (+37.64%). Conclusion: Oral nutritional supplementation used in the study seems promising for reducing the loss of skeletal muscle mass, but further studies involving a larger number of patients are needed to confirm the results. (AU)

RESUMO: Introdução: Os procedimentos maxilofaciais para deformidades esqueléticas são caracterizados por intervenções morfológicas estruturais, necessários por conta de genética desfavorável com diagnóstico feito durante o cresci-mento craniofacial. A cirurgia ortognática requer uma restrição total na mastigação por 60 dias, levando à perda de peso corporal em indivíduos com sobrepeso e obesos, bem como em pacientes bem nutridos. Objetivo: Avaliar o estado nutricional pré e pós-operatório dos pacientes submetidos à cirurgia ortognática. Material e métodos: Trata-se de um estudo de intervenção. O grupo de estudo recebeu suplementação com proteína de soro de leite, L-arginina, L-glutamina e ácido graxo ômega-3 e ambos os grupos foram avaliados em termos de medidas antropo-métricas e bioquímicas. A avaliação da cicatrização de tecidos moles foi realizada após a cirurgia. Resultados: É possível inferir que o percentual de massa muscular esquelética tendeu a aumentar no grupo de intervenção, porém a perda de peso foi maior (-4,88%). O ácido úrico aumentou no pós-operatório no grupo controle (+37,64%). Conclusão: A suplementação nutricional oral utilizada no estudo parece promissora para reduzir a perda de massa muscular esquelética, mas estudos adicionais envolvendo um número maior de pacientes são necessários para confirmar os resultados. (AU)

Acculturation orientations, acculturative stress and psychological well-being on Latin American immigrants settled in Santiago, Chile / Orientaciones de aculturación, estrés de aculturación y bienestar psicológico en inmigrantes latinoamericanos en Santiago de Chile

Abstract In Chile, the significant increase of migratory flows from Latin America has drawn attention to the psychological and socio-cultural adjustment processes of new immigrants. This paper seeks to (i) establish relationships between acculturation orientations, acculturative stress and psychological well-being, (ii) identify profiles according to acculturation preferences, and (iii) determine the existence of significant differences in stress and well-being between these profiles. This correlational study was conducted with a sample of (N=194) Latin American immigrants with ages between 18 and 67 years (M=34.77; DE=10.181), where acculturation orientations, acculturation stress and psychological well-being were evaluated. Results indicate that the predominance of integration is more salutogenic than the tendency to cultural separation as a preeminent orientation, facilitating a better coping with the stressors of the migratory process. Three acculturative profiles were found: moderate individualist, integrationist and separatist. The comparison between profiles shows that when integration is predominant, well-being is greater than in the presence of individualism as the principal orientation. This study provides evidence regarding individualism as an acculturation orientation scarcely considered in the literature, whose possible negative influence can be related to group membership as a way to access resources that collaborate with adaptation to the new context.

Resumen En Chile, el incremento del flujo migratorio desde América Latina ha llamado la atención con respecto a los procesos de ajuste psicológico y sociocultural de los nuevos inmigrantes. Por tanto, con este trabajo se busca (a) establecer relaciones entre las orientaciones de aculturación, el estrés aculturativo y el bienestar psicológico, (b) identificar perfiles según las preferencias de aculturación, y (c) determinar la existencia de diferencias significativas en el estrés y el bienestar, de acuerdo con estas tipologías. Para esto, se realizó un estudio de tipo correlacional con una muestra de (N = 194) inmigrantes latinoamericanos con edades entre los 18 y los 67 años (M = 34.77; DE = 10.181), donde se evaluaron las orientaciones de aculturación, el estrés por aculturación y el bienestar psicológico. Los resultados indican que el predominio de la integración es más salutogénico que la tendencia a la separación cultural como orientación preeminente, lo que facilita un mejor afrontamiento de los estresores del proceso migratorio. Asimismo, se encontraron tres perfiles aculturativos -individualista moderado, integracionista y separatista-, y la comparación entre perfiles arroja que cuando la integración es más saliente, el bienestar es mayor que en presencia del individualismo como orientación predominante. Este trabajo aporta evidencia con respecto al individualismo como orientación de aculturación escasamente considerada en la literatura, cuya posible influencia negativa puede relacionarse con la pertenencia grupal como vía para acceder a recursos que aportan a la adaptación al nuevo contexto.

Molecular prevalence and factors associated with Babesia vogeli infection in dogs in the Cerrado Mato-Grossense region of Brazil / Prevalência molecular e fatores associados à infecção por Babesia vogeli em cães do cerrado Mato-Grossense

ABSTRACT: Canine babesiosis is a common haemoparasitosis in Brazil. Caused by parasites of the genus Babesia, it is transmitted by ixodid ticks and affects domestic and wild canids. The objective of this study was to verify the prevalence of Babesia species (spp.) using molecular methods in dogs living in urban and rural areas of Cuiabá, Mato Grosso State, Brazil, and to identify the main factors associated with infection. A total of 407 samples from 407 dogs were evaluated using a polymerase chain reaction (PCR) technique, among which Babesia species (spp.) was amplified in 10 (2.5%). Although, no statistical association was found among the variables studied (p>0.05), greater positivity was observed in dogs<1 year of age, male sex, those with free access to the street, and the presence of ticks. PCR samples positive for Babesia spp. were submitted to sequencing and compared in GenBank and exhibited a high degree of similarity with Babesia vogeli sequences.

RESUMO: Babesiose canina é uma hemoparasitose comum no Brasil. Causada por parasitos do gênero Babesia, é transmitida por carrapatos ixodídeos e acomete canídeos domésticos e silvestres. O objetivo deste trabalho foi verificar a prevalência molecular da infecção por Babesia spp. em cães residentes em áreas urbanas e rurais do município de Cuiabá, estado de Mato Grosso, Brasil, e relacionar os principais fatores associados à infecção. Para a pesquisa foram avaliados 407 cães usando a PCR. Das 407 amostras analisadas, 10 (2,5%) amplificaram DNA de Babesia spp. Não foi observada associação estatística entre as variáveis pesquisadas (p>0,05), porém observou-se maior positividade em cães com idade inferior a um ano, machos, com livre acesso à rua e com a presença de carrapatos. Amostras positivas nas PCRs para Babesia spp. foram submetidas a sequenciamento e comparadas no GenBank, mostrando alto grau de similaridade com as sequências de B. vogeli.

Population and reproductive traits of a freshwater amphipod (Crustacea, Peracarida, Hyalellidae) from northwest of the state of Rio Grande do Sul, Brazil / Características populacionais e reprodutivas de um anfípodo de água doce (Crustacea, Peracarida, Hyalellidae) do noroeste do estado do Rio Grande do Sul, Brasil

Abstract The study of population and reproductive traits provides information about the ecological structure of natural populations. This study aimed to characterize dynamics and reproductive traits of Hyalella palmeirensis from a natural pond from southern Brazil. The amphipods were sampled monthly (August 2012 to July 2013) by a person with the aid of a hand net for 20 minutes. Ovigerous females and pre-copulatory pairs were individualized in the field. A total of 12,325 individuals were sampled, being 1,421 males, 6,983 females (including 215 ovigerous females) and 3,921 juveniles. Paired and unpaired males were significantly greater in size than females. There was a positive correlation between body size (CL) of paired males and females. Males and females showed bimodal distribution. Total sex ratio favored females, and these were more frequent in almost all months. Ovigerous females and precopulatory pairs were found throughout the year, but with high frequency in winter and autumn, respectively, characterizing a seasonal reproduction. Juveniles were sampled throughout the year, with greater intensity in the spring. The mean fecundity was 19.6 ± 4.34 eggs. No reduction in the number of eggs was observed during embryonic development. The results observed in H. palmeirensis demonstrate that this species has a population and reproductive dynamics very similar to other species of Hyalella already analyzed in southern Brazil. Moreover, it can be seen that although the H. palmeirensis occurs in an environment with anthropic influence (soy cultivation,) the population is managing to remain in the area, with reproduction and recruitment in most months of year.

Resumo O estudo dos aspectos populacionais e reprodutivos providenciam informações sobre a estrutura ecológica das populações naturais. Este estudo teve como objetivo caracterizar aspectos populacionais e reprodutivos de Hyalella palmeirensis de uma lagoa do sul do Brasil. Os anfípodos foram amostrados mensalmente (agosto/2012 a julho/2013) por uma pessoa com o auxílio de puçá por 20 minutos. Fêmeas ovígeras e casais em comportamento pré-copulatório foram individualizados no campo. Um total de 12.325 indivíduos foram amostrados, sendo 1.421 machos, 6.983 fêmeas (incluindo 215 fêmeas ovígeras) e 3.921 juvenis. Machos pareados e não pareados são significativamente maiores em tamanho do que as fêmeas. Houve uma correlação positiva entre o tamanho do corpo (CC) de machos e fêmeas pareados. Machos e fêmeas apresentaram distribuição bimodal. A proporção sexual total favoreceu as fêmeas, e estas foram mais frequentes em quase todos os meses. Fêmeas ovígeras e casais em pré-cópula foram amostrados ao longo do ano, mas com elevada frequência no inverno e outono, respectivamente, caracterizando uma reprodução sazonal. Os juvenis foram amostrados ao longo do ano, com maior intensidade na primavera. A fecundidade média foi de 19,6 ± 4,34 ovos. Não houve redução no número de ovos durante o desenvolvimento embrionário. Os resultados observados em H. palmeirensis demonstram que esta espécie possui uma dinâmica populacional e reprodutiva muito similar a outras espécies de Hyalella já analisadas no sul do Brasil. Além disto, apesar de H. palmeirensis ocorrer num ambiente com influência antrópica (cultivo de soja), sua população está conseguindo se manter na área, com reprodução e recrutamento na maioria dos meses do ano..

Causes of equine abortion, stillbirth, and perinatal mortality in Brazil / Causas de abortamento, natimortalidade e mortalidade em equinos no Brasil

Abortion and complications in reproduction are important causes of economic loss in horse breeding. Studies of its causal agents can help to identify the primary pathogens or other factors involved and define appropriate measures to reduce its occurrence. This research aimed to investigate the primary causes of equine abortion, stillbirth, and perinatal mortality in regions of Brazil. Tissue from aborted fetuses, stillbirths, neonates and foals submitted to the Biological Institute of São Paulo, Brazil, from January 2010 to July 2013 were processed for viral and bacterial isolation, polymerase chain reaction (PCR), histology, and immunohistochemistry. Bacterial infection was the primary detected cause of abortion, found in 16 of the 53 animals submitted for bacterial analysis followed by viruses analysis in 2 of 105 animals, and noninfectious causes (neonatal isoerythrolysis) in 2 of 105 animals. Fungi were found in a single sample of 53 tested. The most frequent bacteria recovered were Escherichia coli, Enterobacter aerogenes, combined E. coli and Streptococcus spp., Staphylococcus spp., and Bacillus spp. The following agents were each observed in a single sample: Arcanobacterium pyogenes, Streptococcus spp., Corynebacterium spp., Actinobacillus spp., and Rhodococcus equi. The predominant identification of fecal and other opportunistic bacteria as opposed to pathogens commonly associated with equine abortion, such as Leptospira spp. and equine herpesvirus type 1 (EHV-1), suggests the need of improving hygiene management of breeding mares to prevent bacterial infection that may cause fetal loss, stillbirth, and perinatal mortality.(AU)

Abortamento e complicações na reprodução são importantes causas de perda econômica na equideocultura. Estudos dos agentes causais podem ajudar a identificar patógenos ou outros fatores envolvidos e definir medidas apropriadas para reduzir sua ocorrência. Esta pesquisa investigou as causas primárias de aborto, natimortalidade e mortalidade perinatal em equinos de diversas regiões do Brasil. Tecidos de fetos abortados, natimortos e potros submetidos ao Instituto Biológico de São Paulo, Brasil, no período de janeiro de 2010 a julho de 2013, foram processados por meio de técnicas de isolamento viral e bacteriano, PCR, histologia e imuno-histoquímica. Infecção bacteriana foi a causa mais detectada, encontrada em 16 de 53 amostras submetidas à análise bacteriana, seguida de causa viral em 2 de 105 amostras, e causas não infecciosas (isoeritrólise neonatal) em 2 de 105 amostras. Fungo foi encontrado em uma única amostra de 53 testadas. As bactérias isoladas mais frequentemente foram Escherichia coli, Enterobacter aerogenes, E. coli associada a Streptococcus spp., Staphylococcus spp. associado a Bacillus spp. Os seguintes agentes foram observados em uma única amostra cada: Arcanobacterium pyogenes, Streptococcus spp., Corynebacterium spp., Actinobacillus spp. e Rhodococcus equi. A identificação predominante de bactérias fecais e outras bactérias oportunistas, ao invés de outros patógenos comumente associados a quadros de abortamento equino, tais como Leptospira spp. e Herpesvírus equino tipo 1, sugere a necessidade de maior atenção no manejo higiênico das éguas em reprodução, a fim de prevenir infecções bacterianas que possam causar perda fetal, natimortalidade e mortalidade perinatal.(AU)

Equidade de gênero intrafamiliar nas narrativas de psicólogas de Centros de Referência de Assistência Social de São Luís do Maranhão / Intrafamilial gender equity in the narratives of psychologists of the Reference Centers of Social Assistance from São Luís do Maranhão / Equidad de género intrafamiliar en las narrativas de psicólogas de los Centros de Referencia de Assistencia Social de São Luís do Maranhão

O artigo analisa as narrativas de psicólogas dos Centros de Referência de Assistência Social de São Luís do Maranhão com o objetivo de entender se e como o trabalho com as questões de gênero é desenvolvido no Serviço de Proteção e Atendimento Integral à Família. A partir de referencial teórico dos estudos feministas, de gênero e da psicologia social, foram realizadas e analisadas entrevistas semiestruturadas com cinco profissionais. Os resultados indicam que, embora as questões de gênero atravessem a prática no Serviço, o Trabalho Social realizado foca principalmente em demandas socioeconômicas emergenciais. As entrevistadas trouxeram questões práticas que podem ser pensadas a partir dos estudos de gênero e feministas, mas relataram não possuir ferramentas técnicas e teóricas para abordá-las

This article analyzes the narratives of psychologists from the Social Assistance Referral Centers (CRAS) of São Luís do Maranhão in order to understand if, and how, work with gender issues is developed in the Comprehensive Protection and Care Service Family (PAIF). Based on the theoretical framework of the feminist, gender and social psychology studies, semi-structured interviews were carried out with five professionals. The results indicate that although the gender issues cross the practice in the Service, the Social Work carried out focuses mainly on emergency socioeconomic demands. The interviewees brought practical questions that could be thought from gender and feminist studies , but they reported not having the technical and theoretical tools to approach them

El artículo analiza las narrativas de psicólogas de los Centros de Referencia de Asistencia Social (CRAS) de São Luís de Maranhão con el objetivo de entender si, y cómo, se desarrolla el trabajo con las cuestiones de género en el Servicio de Protección y Atención Integral Familia (PAIF). A partir de referencial teórico de los estudios feministas, de género y de la psicología social, se realizaron y analizaron entrevistas semiestructuradas con cinco profesionales. Los resultados indican que, aunque las cuestiones de género atraviesan la práctica en el Servicio, el Trabajo Social realizado se centra principalmente en demandas socioeconómicas de emergencia. Las entrevistadas trajeron cuestiones prácticas que pueden ser pensadas a partir de los estudios de género y feministas, pero relataron no poseer herramientas técnicas y teóricas para abordarlas

Efectos agudos del ejercicio resistido en la lipemia postprandial de mujeres postmenopáusicas con sobrepeso / Acute effects of resistance training on postprandial lipemia in overweight postmenopausal women

Resumen Introducción. La magnitud de la lipemia postprandial es un indicador de riesgo cardiovascular, en especial en mujeres con actividad hormonal reducida. Los ejercicios resistidos (ER) podrían ser un factor que influencie las concentraciones de lípidos después de consumir alimentos. Objetivo. Determinar los efectos agudos sobre la lipemia postprandial de mujeres postmenopáusicas que tiene la realización de sesiones de ER de alto y bajo volumen, en comparación a un grupo control. Materiales y métodos. Después de una evaluación inicial (antropometría, perfil lipídico y fitness muscular), 32 mujeres fueron divididas aleatoriamente en tres grupos: uno con ER de alto volumen (n=11), otro con ER de bajo volumen (n=11) y un grupo control sin ER (n=10). 12 horas después de los ejercicios se suministró un compuesto nutricional hiperlipídico y se analizó la lipemia postprandial cada hora durante 5 horas. Resultados. Los diferentes volúmenes de ER no redujeron de manera importante los marcadores lipémicos (colesterol total, triglicéridos, LDL y VLDL) (p>0.05) ni aumentaron las concentraciones plasmáticas de HDL (p>0.05). Conclusiones. Las sesiones de ER no afectaron los marcadores lipémicos postprandiales, aunque sí mostraron resultados clínicos relevantes en los grupos experimentales (reducción de LDL-VLDL y aumento de HDL en momentos específicos).

Abstract Introduction: The magnitude of postprandial lipemia is an indicator of cardiovascular risk, especially in women with reduced hormonal activity. Resistance training (RT) may be a factor influencing lipid concentrations after eating. Objective: To determine the acute effects of high and low volume RT sessions on postprandial lipemia in postmenopausal women compared to a control group. Materials and methods: After an initial assessment (anthropometry, lipid profile and muscle fitness), 32 women were randomly divided into three groups: one with high volume of RT (n=11), another with low volume of RT (n=11) and a control group without RT (n=10). 12 hours after the completion of the training, a hyperlipidic nutritional compound was administered and postprandial lipemia was analyzed every hour for 5 hours. Results: The different RT volumes did not significantly reduce lipemic markers (total cholesterol, triglycerides, LDL and VLDL) (p>0.05) nor did they increase plasma HDL concentrations (p>0.05). Conclusions: RT sessions did not affect postprandial lipemic markers, although they did show relevant clinical results in the experimental groups (reduction of LDL-VLDL and increase of HDL at specific times).

Occurrence of antibodies against Leptospira spp. in donkeys of São Paulo state / Ocorrência de anticorpos contra Leptospira spp. em asininos no estado de São Paulo

Among the diseases that affect equines, bacterial diseases play an important role from a health and economic point of view, especially leptospirosis and brucellosis. The study aimed to provide information on the occurrence of anti-Leptospira spp. and anti-Brucella abortus antibodies in donkeys of São Paulo state. We found a frequency of 62.4% (53/85) antibodies against Leptospira spp. The donkeys were not seropositive for Brucella spp.(AU)

Entre as doenças que acometem os equídeos, as enfermidades bacterianas assumem um papel importante do ponto de vista sanitário e econômico, destacando-se a leptospirose e a brucelose. O estudo teve como objetivo fornecer informações sobre a ocorrência de anticorpos anti-Leptospira spp. e anti-Brucella abortus em jumentos no estado de São Paulo. Estimou-se que 62,4% (53/85) dos animais apresentavam anticorpos anti-Leptospira spp. Os jumentos estudados não foram sororreagentes contra a Brucella spp.(AU)

Análise sociodemográfica e ambiental para ocorrência de anticorpos antiLeptospira em cães de Teresina, Piauí, Brasil / Sociodemographic and environmental analysis for the occurrence of anti-Leptospira antibodies in dogs of Teresina, Piauí, Brazil

Resumo A leptospirose é uma zoonose mundial cuja transmissão está interligada por múltiplos fatores na interface homem-animal-ecossistema. Objetivou-se com este estudo avaliar os fatores de risco para a ocorrência de anticorpos antiLeptospira em cães na capital Teresina (PI), e determinar sua distribuição espacial. Amostras sanguíneas de 558 cães foram submetidas à prova de Soroaglutinação Microscópica (SAM). Aplicou-se questionários semiestruturados para os proprietários dos cães e obteve-se a localização geográfica da residência para a sua projeção em mapas geográficos. A soroprevalência foi de 13,8%, no qual o sorogrupo mais frequente foi o Icterohaemorrhagiae com 49,2%. Foram considerados fatores de risco os cães com acesso à rua, o não recolhimento da vasilha de alimento e a baixa renda dos proprietários. Foi observado maior número de cães soropositivos no período chuvoso com 87,1%, sendo um possível fator de risco para a ocorrência de casos. A distribuição dos cães soropositivos na cidade se apresentou de forma dispersa, com predominância dos casos em área antropizada. Esses fatores de risco favorecem a ocorrência de anticorpos antiLeptospiraem cães, os quais podem ser fontes de manutenção do agente na cidade e reforça a necessidade de vigilância epidemiológica e ambiental na prevenção da leptospirose.

Abstract Leptospirosis is a worldwide zoonosis whose transmission is interlinked by multiple factors in the man-animal-ecosystem interface. This study aimed to evaluate the risk factors for the occurrence of anti-Leptospira antibodies in dogs in the capital Teresina (PI), and to determine their spatial distribution. Five hundred fifty-eight dog blood samples were submitted to the Microscopic Serum Agglutination (MSA) test. We applied semi-structured questionnaires to dog owners and obtained the area of residence for projection in geographical maps. Serum prevalence was 13.8%, in which the most common serovar was icterohaemorrhagiae, with 49.2%. Dogs with street access, failure to collect food bowl and low income of owners were risk factors. There was a higher number of seropositive dogs in the rainy season, with 87.1%, which is a probable risk factor for the occurrence of cases. The distribution of seropositive dogs was widely spread in the city, with predominance of cases in anthropized areas. These risk factors favor the occurrence of anti-Leptospira antibodies in dogs that are agent maintenance sources in the city and reinforce the need for epidemiological and environmental surveillance to prevent leptospirosis.

Serological survey of anti-Leptospira spp. antibodies in Barbary sheep (Ammotragus lervia) at the Curitiba Zoo, southern Brazil / Estudo sorológico de anticorpos anti-Leptospira spp. em aoudads (Ammotragus lervia) no zoológico de Curitiba, sul do Brasil

Leptospirosis is a worldwide zoonosis, affecting humans, domestic and wild animals. The present study aimed to evaluate prevalence of anti-Leptospira spp. antibodies in Barbary sheep at the Curitiba zoo. Microscopic agglutination test (MAT) was performed using 17 serogroups. Antibodies against Leptospira spp. were observed in 23.5% samples and Icterohaemorrhagiae was the only prevalent serogroup. The presence of anti-Leptospira antibodies in Barbary sheep indicates exposure to leptospires; thus monitoring and preventive measures are necessary in zoo's captive animals, since they can act as sentinels of environmental exposure in an area with high movement of people.(AU)

A leptospirose é uma zoonose mundial que afeta seres humanos, animais domésticos e selvagens. O presente estudo objetivou avaliar a prevalência de anticorpos anti-Leptospira spp. em aoudads do zoológico de Curitiba. Foi realizado o teste de Soroaglutinação microscópica (SAM) utilizando 17 sorogrupos. Anticorpos contra Leptospira spp. foram observados em 23.5% das amostras de aoudads e Icterohaemorrhagiae foi o único sorogrupo prevalente. A presença de anticorpos em aoudads indica exposição a leptospiras portanto monitoramento e medidas preventivas são necessários em animais confinados em zoológicos, uma vez eles podem atuar como sentinelas de exposição ambiental em uma área com alta circulação de pessoas.(AU)

Efectos agudos del ejercicio resistido sobre los niveles de Lecitin Colesterol Acil Transferasa en algunas mujeres postmenopáusicas de la ciudad de Barranquilla (Colombia) / Acute effects of the resistance exercise on lecithincholesterol acyltransferase levels in some postmenopausal women of Barranquilla (Colombia)

Objetivo: determinar el efecto agudo que tiene sobre los niveles séricos de Lecitin Colesterol Acil Transferasa de mujeres postmenopáusicas la realización de una sesión de ejercicios resistidos, comparados con un grupo control sin ejercicios. Materiales y métodos: dos grupos de postmenopáusicas asignadas al azar (n=10), un grupo realizó una sesión de ejercicios resistidos (8 ejercicios, 3 series a 15 repeticiones) y un grupo control con ejercicios de estiramientos. Inicialmente fueron evaluadas las características antropométricas, funcionales y el perfil lipídico de ambos grupos, en el protocolo experimental se evaluó las concentraciones sanguíneas de HDL y Lecitin Colesterol Acil Transferasa basales en ayunas, antes, inmediatamente y 12 horas después del ejercicio. Se realizó un control nutricional antes y durante la realización del experimento. Resultados: no hubo diferencias en ninguna de las variables antropométricas,nutricionales y del perfil lipídico antes del protocolo experimental (P>0,05).Comparando las medias de los grupos, no se encontraron diferencias estadísticas en ningún momento de evaluación de las concentraciones de HDL y Lecitin Colesterol Acil Transferasa (P>0,05). Conclusión: de forma aguda, los ejercicios resistidos no mostraron influencia sobre las concentraciones sanguíneas de Lecitin Colesterol Acil Transferasa en mujeres postmenopáusicas..(AU)

Objective: determine the acute effect of performing resistance exercise on serum concentrations of Lecithin Cholesterol Acyl-Tranferase in postmenopausal women. Materials and methods: two groups of postmenopausal women were randomized (n=10). The experimental group performed a resisted exercise session (8 exercises, 3 sets to 15 repetitions) and the control group performed a stretching exercises session.Anthropometrics characteristics, functional and lipid profile were initially evaluated in both groups. In the experimental protocol, blood concentrations of HDL and Lecithin Cholesterol Acyl-Tranferase were measured in fasted state, before, immediately and 12 hours after exercise. The participants were nutritionally controlled before and during the study. Results: there were no significant differences in anthropometrics variables, nutritionals, and lipid profile previous to the experimental protocol (P>0.05). No statistically significant differences were found on blood levels of HDL and Lecithin Cholesterol Acyl-Tranferase between control and experimental group throughout the evaluation (P>0.05). Conclusion: resistance exercise don't affected acutely, serum concentrations of Lecithin Cholesterol Acyl-Tranferase in postmenopausal women..(AU)

As atribuições da Educação Física escolar: um enfoque especial no Ensino Médio / The attributions of school Physical Education: a special focus on High School / Las atribuciones de la Educación Física escolar: una mirada especial al Enseño Medio

Trata-se de uma breve reflexão sobre as atribuições da Educação Física escolar ao longo do século XX e início do século XXI, com enfoque especial no ensino médio. O objetivo principal é pontuar de forma crítica, com base em documentos legais, a consolidação da educação básica no país e as atribuições da Educação Física escolar nesse processo, principalmente no ensino médio. Ao longo do século XX, a educação básica se consolidou no Brasil, e a Educação Física escolar se tornou um componente curricular obrigatório, tendo seu papel bem definido pela legislação pertinente. Nas primeiras décadas do século XXI, sua obrigatoriedade curricular passou a ser questionada, especialmente no novo ensino médio, dados os próprios problemas dessa etapa crucial da educação básica, que acaba de passar por uma reforma estrutural e curricular. Conclui-se que as atribuições da Educação Física escolar estão vinculadas ao contexto no qual essas atribuições são forjadas.

This paper is a brief reflection about the attributions of school physical education through the 20th and beginning of the 21st centuries, with a special focus on high school. The main objective is to analyze critically, based on legal documentation, the consolidation of basic education in Brazil and the attributions of physical education in said process. It was along the 20th century that basic education consolidated in Brazil and physical education became a mandatory curricular component, with a role well defined by legislation. In the first decades of the 21st century the mandatory aspect began to be questioned, especially in the new high school, given the problems afflicting that stage of basic education that underwent a curricular and structural reform. The conclusion states that the attributions of physical education are bounded to the context in which they are forged.

Este trabajo es una pequeña reflexión acerca de las atribuciones de la educación física a través del siglo XX e comenzó del XXI, con una mirada especial en el enseño medio. El objetivo central es analizar críticamente, con base en documentos legales, la consolidación de la educación básica en Brasil y las atribuciones de la educación física en este proceso. Fue al largo del siglo XX que la educación básica se consolidó en Brasil y la educación física quedó como un componente curricular obligatorio, con un papel bien definido por la legislación. En las primeras décadas del siglo XXI el aspecto obligatorio de la disciplina empezó a ser cuestionado, especialmente en el enseño medio, frente a los problemas de esta etapa de la educación básica, objeto de una reciente reforma curricular y estructural. Las conclusiones apuntan que las atribuciones la educación física están directamente conectadas al contexto en que fueran criadas.

Efectos del atractivo físico y el sexo en la selección de personal: Un estudio experimental / Effects of Sex and Attractiveness in Hiring Decisions: An Experimental Study

RESUMEN El presente estudio tuvo como objetivo analizar la activación de información estereotípica en contextos de selección de personal. Las hipótesis fueron contrastadas mediante un diseño experimental 2 (sexo del aplicante) x 2 (atractivo del aplicante) x 2 (sexo de la persona reclutadora) en un grupo de reclutadores profesionales. Los resultados indicaron un efecto significativo tanto del sexo como del atractivo de los estímulos. Estos efectos fueron moderados por el sexo de las personas reclutadoras y parcialmente mediados por las atribuciones de calidez y habilidad. Los resultados se discuten considerando el impacto del estereotipo de "lo bello es bueno" y los estereotipos sobre los roles tradicionales asignados a cada sexo.

ABSTRACT This study aimed to analyze the activation of stereotypical information in personnel selection contexts. Hypotheses were tested via a 2 (applicant's sex) x 2 (applicant's appeal) x 2 (sex of the recruiter) experimental design among professional recruiters. Results show a significant effect of both, sex and attractiveness of the stimuli. These effects were moderated by the sex of the recruiter and partially mediated by attributions of warmth and ability. Results are discussed in the light of the impact of the "beautiful is good" effect and the stereotypes of traditional sex roles.

Frequency of and risk factors associated to Leptospira spp. Seropositivity in goats in the state of Sergipe, Northeastern Brazil / Frequência e fatores de riscos associados a caprinos soropositivos a Leptospiras spp. no Estado de Sergipe, Nordeste do Brasil

ABSTRACT: The objective of this study was to determine the frequency and factors associated with the occurrence of seropositivity for Leptospira spp. in goat herds in the state of Sergipe, Brazil, on the basis of the analysis of 675 samples collected from 41 properties in 2013-2014. Analysis of anti-Leptospira spp. antibodies revealed that 25.74% goats (194) were seropositive and that 90.24% (37) of the properties had at least one infected goat. The most prevalent serogroup was Icterohaemorrhagiae (85.57%); although, the study animals were reactive to other serogroups: Australis (5.15%), Pomona (3.61%), Sejroe (3.09%), and Pyrogenes (2.58%) had titers ≤400 in 96.91% of cases. The origin of the water source [still water source (OR=3.86)] was associated with seropositivity. Results reflected the importance of adopting appropriate management practices for herds in Sergipe.

RESUMO: O objetivo deste estudo foi determinar a frequência e os fatores associados à soropositividade para Leptospira spp. em caprinos do Estado de Sergipe, Nordeste do Brasil, utilizando 675 animais de 41 propriedades no período de 2013 e 2014. A frequência de positividade entre os animais foi de 25,74% (194), e em 90,24% (37) das propriedades houve pelo menos um animal soropositivo. O sorogrupo reagente predominante foi o Icterohaemorrhagiae (85,57%), com presença também de animais reagentes para Australis (5,15%), Pomona (3,61%), Sejroe (3,09%) e Pyrogenes (2,58%) com títulos ≤400 em 96,91% dos casos. A origem da água fornecida (água parada) (OR=3,86) foi o fator associado à presença de caprinos positivos. Ressalta-se a importância da adoção de práticas de manejo adequadas nos rebanhos caprinos sergipanos.